《植物生理学报》 2018, 54(2): 335-345

杜鹃花品种耐热性评价及其生理机制研究

申惠翡^1,2, 赵冰^1,*

¹西北农林科技大学风景园林艺术学院, 陕西杨凌712100; ²太原市晋阳湖管理处, 太原030000

通信作者：赵冰；E-mail: Bingbing2003915@163.com

摘　要：

以15个杜鹃花品种为试验材料, 在人工模拟高温胁迫条件下测定11项生理指标。结果发现: 高温胁迫条件下, 大部分品种的叶绿素a、b含量、总叶绿素含量和类胡萝卜素含量下降, 相对电导率增加, 而MDA含量、H₂O₂含量、可溶性蛋白含量、Pro含量、SOD和POD活性的变化因品种而异。通过主成分及相关性分析发现: 叶绿素、MDA、可溶性蛋白、Pro和H₂O₂含量与杜鹃花耐热性联系紧密, 可作为鉴定不同品种杜鹃花耐热性的依据。经过隶属函数分析综合评价, 15个杜鹃花品种耐热性顺序为: ‘松江大桃红’>‘粉珍珠’>‘爱丁堡’>‘套瓣朱砂’>‘绿色光辉’>‘大和之春’>‘状元红’>‘外国红’>‘蓝茵’>‘国旗红’>‘琉球红’>‘玉玲珑’>‘笔止’>‘红珊瑚’>‘宁波红’。

关键词：杜鹃花; 耐热性; 生理特性; 隶属函数法

收稿：2017-05-23   修定：2018-01-24

资助：西北农林科技大学基本科研业务费项目(Z109021703)和西北农林科技大学国际科技合作种子基金(A213021701)。

Study on evaluation of heat tolerance and its physiological mechanisms in Rhododendron cultivars

SHEN Hui-Fei^1,2, ZHAO Bing^1,*

¹The College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi 712100, China; ²The Management of Taiyuan Jinyang Lake, Taiyuan 030000, China

Corresponding author: ZHAO Bing; E-mail: Bingbing2003915@163.com

Abstract:

To analyze the heat tolerance in 15 Rhododendron cultivars, under artificial simulation of heat stress, 11 physiological indexes were measured. The results showed that chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents decreased and relative electrolytic leakage increased in most cultivars. However, the changes of MDA, H₂O₂, total soluble protein and proline contents, the SOD and POD activites were different from cultivar to cultivar. Correlation and principal component analysis showed that the contents of chlorophyll, MDA, soluble protein, Pro and H₂O₂ were closely related to the heat resistance of Rhododendron, which could be used as identification indexes in heat resistance. Using subordinate function method to comprehensively evaluate the heat tolerance in 15 Rhododendron cultivars, which was ‘Song Jiang Da Tao Hong’ >‘Fen Zhen Zhu’ > ‘Ai Ding Bao’ > ‘Tao Ban Zhu Sha’ > ‘Lv Se Guang Hui’ >R. ‘Da He Zhi Chun’ > ‘Zhuang Yuan Hong’ > ‘Wai Guo Hong’ > ‘Lan Yin’ > ‘Guo Qi Hong’ >‘Liu Qiu Hong’ > ‘Yu Ling Long’ >. ‘Bi Zhi’ > ‘Hong Shan Hu’ >‘Ning Bo Hong’.

Key words: Rhododendron; heat tolerance; physiological characters; subordinate function (SF) method